Genetic Engineering and rDNA Technology

Genetic engineering is a broad term referring to manipulation of an organisms’ nucleic acid. Organisms whose genes have been artificially altered for a desired affect is often called genetically modified organism (GMO). Recombinant DNA technology (rDNA) is technology that is used to cut a known DNA sequence from one organism and introduce it into another organism thereby altering the genotype (hence the phenotype) of the recipient. The process of introducing the foreign gene into another organism (or vector) is also called cloning. Sometimes these two terms are used synonymously.

Basically, these techniques are used to achieve the following are: Study the arrangement, expression and regulation of genes, Modification of genes to obtain a changed protein product, Modification of gene expression either to enhance or suppress a particular product, Making multiple copies of a nucleic acid segment artificially, Introduction of genes from organism to another, thus creating a transgenic organism, Creation of organism with desirable or altered characteristics.

  • Recombinant DNA technology and cloning
  • Genome sequencing and next generation sequencing
  • Human genetic engineering and genome research
  • Genetics syndromes and gene therapy
  • Gene editing technology and CRISPR/Cas9
  • Human moleculer cytogenetics
  • Genomics of model organism

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